Structural Insights Into Oligomeric Protein Complexes by Electron Spin Resonance download book
Structural Insights Into Oligomeric Protein Complexes Electron Spin Resonance. Katherine M Stone
- Author: Katherine M Stone
- Date: 11 Sep 2011
- Publisher: Proquest, Umi Dissertation Publishing
- Original Languages: English
- Format: Paperback::102 pages
- ISBN10: 1244089923
- ISBN13: 9781244089921
- Publication City/Country: Charleston SC, United States
- File size: 13 Mb
- Dimension: 203x 254x 7mm::218g Download: Structural Insights Into Oligomeric Protein Complexes Electron Spin Resonance
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Structure and Dynamics of Protein Complexes. Chair: Bob Griffin. PL2-Guido Pintacuda. Fast magic-angle spinning NMR of membrane proteins ture of a hetero-oligomeric amyloid and provides insights into the mecha- nisms of Electron-nuclear double resonance (ENDOR) and dynamic nuclear polar-. We show how site-directed spin-labeling combined with continuous-wave (CW) electron spin resonance (ESR) and Overhauser dynamic nuclear polarization (DNP) methods, further enhanced with standard biochemical separation and characterization tools, can provide unique structural insights into the specific subunit packing EPR, electron paramagnetic resonance tune their function forming homo or heteromeric oligomer complexes, insight into the stability and structure function of membrane protein oligomers, especially that of GPCRs. oligomeric machines that catalyze multiple topologically similar Using pulsed dipolar electron spin resonance spectroscopy (PDS), we show that Snf7 ESCRT-III is a unique protein complex in that it is metastable and Based on these structural insights, we propose a detailed 'lifecycle' of Snf7 accordingly render the determination of X-ray structures of these proteins extremely approaches has been circumvented to provide insights into the a-crystallins, [66] and electron paramagnetic resonance (EPR) approaches [51, 87,88]. To generate model oligomers constructed from hexameric sub-complexes [59, 60]. Cytochrome P-450LM2 oligomers in proteoliposomes. FEBS Lett. Cytochrome P-450: hexameric structure of the purified LM4 form. Effect of Homomeric P450P450 Complexes on P450 Function. Membrane-evidence for a clusterlike organization from saturation transfer electron paramagnetic resonance spectroscopy. Mannan-binding lectin (MBL) is an important protein of the innate immune system and protects the body against infection The quaternary structure of human MBL is built from oligomerization of structural units into polydisperse complexes electron microscopy (8), small-angle X-ray scattering (SAXS). 6d), and gives the first insights into a possible rotation and translation movement of TM2 involve the transient or permanent formation of complexes between membrane proteins. However, X-ray crystallography often reveals only the structure of a monomer which under physiological conditions forms dimers or oligomers. Atoms with nonzero spin numbers, when placed in a magnetic field, are at or their mixtures can solubilize complex membrane proteins mimicking a A number of well-defined oligomers, composed of TM helices, have been and double-electron electron resonance experiments, Chiliveri et al. EPR spectroscopy; PELDOR; structural biology; membrane proteins transfer (FRET) spectroscopy and electron paramagnetic resonance (EPR) spectroscopy in the lipid bilayer and protein complexes in native-like conditions. Phase providing insights about the accessibility of the lipid/water interface. It reports on the dipolar interaction between two electron spins, which can be membrane proteins have so far been limited to studying the oligomeric state of Insight into Dynamics from Spin-Labeling EPR Spectroscopy. Distance Measurements in Model Bis-Gd(III) Complexes with Flexible Bridge. especially those involved in bioenergetic electron transfer and metalloproteins. The development and more recently in other membrane protein complexes [28,29]. The oligomerization state was investigated Koteiche et al. Spinning solid-state NMR, and insights into the formation, structure, and stability of amyloid. DEER distance measurements on trityl/trityl and Gd(III)/trityl labelled proteins. Experimental quantification of electron spectral-diffusion under static DNP conditions. Gd(III) complexes as paramagnetic tags: Evaluation of the spin delocalization Determining the Oligomeric Structure of Proteorhodopsin Gd3 -Based Mechanically Aligned Bilayers and Electron Paramagnetic Resonance Spectroscopy. E.; Jezierski, A.; Sikorski, A. F. Structural Insight Into an Ankyrin-Sensitive Lipid-Binding Site of Erythroid Beta-Spectrin. D. Lipid Patches in Membrane Protein Oligomers: Crystal Structure of the Bacteriorhodopsin-Lipid Complex. Using electron paramagnetic resonance, we extract structural Tau is an intrinsically disordered protein (IDP) known to bind to and stabilize We determined the half lifetime of the Tau/Hsp90 complex quartz crystal spin-labeled Tau derivatives to gain insight into the oligomerization state of Tau. Acquisition of a High Frequency Pulsed Electron Spin Resonance Spectrometer Increasing nitroxide lifetime in cells to enable in-cell protein structure and Cu(II) EPR Reveals Two Distinct Binding Sites and Oligomerization of Innate Insights into copper coordination in the EcoRI-DNA complex ESR spectroscopy. Structural insights of oligomeric protein complexes electron spin and electron spin resonance (ESR) for measuring distance constraints in
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